Fig. 2 . ( B ) Experiment 2 refers to
. ( C ) Experiment 3 refers to
and . ( D ) Representative image of co-culture setup at time of plating containing human cumulus–oocyte complexes (COCs) (n = 5) and 100 000 ovarian support cells (OSCs). Scale bar: 100 µm. COCs with varying degrees of cumulus enclosure are seen with surrounding OSCs in suspension culture. COCs, cumulus–oocytes complexes; ICSI, intracytoplasmic sperm injection; IU, international units; rFSH, recombinant follicle stimulating hormone; Clomid, clomiphene citrate; hCG, human chorionic gonadotropin; IVM, in vitro maturation; LAG, pre-incubation medium (MediCult); PGT-A, pre-implantation genetic testing for aneuploidy; EM-Seq; enzymatic methylation sequencing. " width="100%" height="100%">
Journal: Human Reproduction (Oxford, England)
Article Title: Human-induced pluripotent stem cell-derived ovarian support cell co-culture improves oocyte maturation in vitro after abbreviated gonadotropin stimulation
doi: 10.1093/humrep/dead205
Figure Lengend Snippet: Study design flow chart. Schematic representation of the three independent experiments performed in the study, depicting stimulation protocols and embryology workflows. ( A ) Experiment 1 refers to Fig. 2 . ( B ) Experiment 2 refers to Fig. 3 . ( C ) Experiment 3 refers to Figs 4 and . ( D ) Representative image of co-culture setup at time of plating containing human cumulus–oocyte complexes (COCs) (n = 5) and 100 000 ovarian support cells (OSCs). Scale bar: 100 µm. COCs with varying degrees of cumulus enclosure are seen with surrounding OSCs in suspension culture. COCs, cumulus–oocytes complexes; ICSI, intracytoplasmic sperm injection; IU, international units; rFSH, recombinant follicle stimulating hormone; Clomid, clomiphene citrate; hCG, human chorionic gonadotropin; IVM, in vitro maturation; LAG, pre-incubation medium (MediCult); PGT-A, pre-implantation genetic testing for aneuploidy; EM-Seq; enzymatic methylation sequencing.
Article Snippet: 75 mIU/ml of recombinant FSH (Millipore, F4021) , X , X , X , X , X , X.
Techniques: Co-Culture Assay, Suspension, Injection, Recombinant, In Vitro, Incubation, Methylation, Sequencing